| dc.description.abstract |
To clarify the cause(s) of the differences between the developmental competence of prepubertal and adult porcine oocytes, the following were examined: (i) the meiotic progression, p34<emph type="7">cdc2 kinase activity, ooplasm diameter and response to activation stimuli of the oocytes; and (ii) the development of parthenotes and nuclear transfer (NT) embryos obtained using oocytes recovered from prepubertal and adult pigs. Oocytes were recovered from 4- to 8-mm follicles of abattoir-derived ovaries. There were no apparent differences in the morphology of the germinal vesicle, nuclear maturation rate, activity of p34<emph type="7">cdc2 kinase or response to parthenogenetic stimulation between prepubertal and adult oocytes. Before and after maturation culture, the ooplasm diameters of prepubertal oocytes were smaller than those of adult oocytes. Parthenotes and NT embryos derived from prepubertal gilt oocytes showed a lower rate of development to the blastocyst stage than those derived from adult oocytes. These results suggest that lower developmental competence of prepubertal oocytes may be caused by their inability to complete ooplasmic maturation, and that this is not because of altered oocyte maturation kinetics and/or p34<emph type="7">cdc2 kinase activity. Furthermore, the smaller diameter of the ooplasm of prepubertal oocytes indicates that most oocytes that have routinely recovered from prepubertal gilt ovarian follicles are still in the growing phase and have immature ooplasm. |
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