210 Effects of interferon-T on replication of bovine viral diarrhea virus and bovine herpesvirus-1.

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dc.contributor Galik, P
dc.contributor Waldrop, J
dc.contributor Marley, S
dc.date.accessioned 2012-01-31T12:52:10Z
dc.date.available 2012-01-31T12:52:10Z
dc.date.issued 2006
dc.identifier.citation Rep. Fert. Dev. (2006) 18(1&2): 213-213
dc.identifier.issn 1031-3613
dc.identifier.uri http://livestocklibrary.com.au/handle/1234/18538
dc.description.abstract Bovine viral diarrhea virus (BVDV) and bovine herpesvirus-1 (BHV-1) are the most likely viruses to be associated with abattoir-origin materials used in in vitro embryo production. Further, both viruses are known to associate with zona pellucida-intact embryos after exposure and washing, and limited evidence indicates that developing, transferable embryos are able to inhibit viral replication in adjacent cells. Interferon-τ is known to have anti-BVDV and anti-BHV-1 activities, but it is not known whether interferon-τ which is secreted by developing embryos has the same effects. The objectives of this study were to evaluate the cytotoxicity and anti-viral effect of interferon-τ against a non-cytotoxic high affinity strain of BVDV (SD-1) and against a strain of BHV-1 (Colorado) in cell culture. Madin Darby bovine kidney (MDBK) cells were seeded in 96-well plates and then inoculated with serial dilutions (1:10) beginning with an initial concentration of 0.2 �g of interferon-τ. Cells and interferon were incubated at 37.5�C in 5% CO2 and air for 24 h prior to addition of virus. Five concentrations of BVDV were added to the wells to give 500, 50, 25, 10 or 5 cell culture infective doses (50% endpoint) per well. Three concentrations of BHV-1-50, 10, and 5 plaque-forming units were evaluated in separate cell cultures. Virus isolation (for BVDV) or plaque assays (for BHV-1) were utilized to determine if the addition of interferon-τ decreased the amount of infective virus. The interferon-τ produced no observable cytotoxicity in MDBK cells in any of the assays. At its three highest concentrations, the interferon-τ significantly decreased the amount of BVDV but it had no significant effect on the amount of BHV-1 in cell cultures. Thus, it is possible that interferon-τ produced by developing embryos might limit or prevent transmission of BVDV to recipients if this virus were to be inadvertently associated with the embryos that are transferred. However, a similar effect is not expected for BHV-1.
dc.publisher CSIRO Publishing
dc.source.uri http://www.publish.csiro.au//nid/44/paper/RDv18n2Ab210.htm
dc.title 210 Effects of interferon-T on replication of bovine viral diarrhea virus and bovine herpesvirus-1.
dc.type Research
dc.description.version Abstract
dc.identifier.volume 18
dc.identifier.page 213-213
dc.identifier.issue 1&2


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